Anti-Poly(A) RNA polymerase, mitochondrial抗体(ab78618)

概述

  • 产品名称Anti-Poly(A) RNA polymerase, mitochondrial抗体
    参阅全部 Poly(A) RNA polymerase, mitochondrial 一抗
  • 描述
    兔多克隆抗体to Poly(A) RNA polymerase, mitochondrial
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Poly(A) RNA polymerase, mitochondrial .

    (Peptide available as ab93076.)

  • 阳性对照
    • This antibody gave a positive signal in Human Skeletal Muscle Tissue Lysate; HepG2 Whole Cell Lysate and HEK293 Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab78618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
  • 组织特异性Ubiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle.
  • 疾病相关Defects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4) [MIM:613672]. A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Note=Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105).
  • 序列相似性Belongs to the DNA polymerase type-B-like family.
    Contains 1 PAP-associated domain.
  • 细胞定位Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 0610027A18Rik antibody
    • AW551379 antibody
    • EC 2.7.7.19 antibody
    • FLJ10486 antibody
    • mitochondrial antibody
    • Mitochondrial poly(A) polymerase antibody
    • mtPAP antibody
    • PAP antibody
    • PAP-associated domain-containing protein 1 antibody
    • PAPD1 antibody
    • PAPD1_HUMAN antibody
    • Poly(A) RNA polymerase antibody
    • Poly(A) RNA polymerase, mitochondrial antibody
    • Poly(A) RNA polymerase, mitochondrial precursor antibody
    • Polynucleotide adenylyltransferase antibody
    • RP11-305E6.3 antibody
    • Terminal uridylyltransferase 1 antibody
    • TUTase 1 antibody
    see all

Anti-Poly(A) RNA polymerase, mitochondrial antibody 图像

  • All lanes : Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab78618) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,120 kDa,150 kDa,30 kDa,35 kDa,45 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • ICC/IF image of ab78618 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab78618 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Poly(A) RNA polymerase, mitochondrial antibody (ab78618)参考文献

ab78618 has not yet been referenced specifically in any publications.

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