Other Immunogen Type conjugated to bovine serum albumin. Poly (ADP-Ribose) polymer mixed with methylated bovine serum albumin.
Our Abpromise guarantee covers the use of ab14459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 2 - 10 µg/ml. 2 µg/ml, if using ECL or 10 µg/ml, if using colorimetric methods. | |
ELISA | Use at an assay dependent concentration. | |
ICC/IF | 1/400. | |
IHC-Fr | Use at an assay dependent concentration. | |
Flow Cyt | Use at an assay dependent concentration. ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
ab14459 staining Poly (ADP-Ribose) Polymer in Rat cardiomyoblast (H9c2) by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with 0.1% Triton X-100. The sample was incubated with the primary antibody (1/1000 in 3% BSA) for 1 hour at 37°C. ab98707, FITC-conjugated Goat anti-mouse FITC (IgG3 heavy chain preadsorbed 1/1000) was used as the secondary antibody.
ab14459 staining Poly (ADP-Ribose) Polymer in human breast cancer cells (MCF7 cells) by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.2% Triton/ PBS and then blocked using 1% BSA and 3% FBS in PBS for 30 minutes at room temperature. Samples were then incubated with primary antibody at 1/50 for 1 hour. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 555 (red) used at a 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"