概述

  • 产品名称
    Anti-Poly A polymerase抗体
  • 描述
    小鼠多克隆抗体to Poly A polymerase
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Fusion protein corresponding to Drosophila melanogaster Peroxidase anti-Peroxidase complex/ PAP aa 18-117.
    Sequence:

    STSGGPPAKQLGMTSAISLAEPRPEDLQRTDELRGSLEPYNVFESQDELN HRMEILAKLNTLVKQWVKEISVSKNMPESAAEKLGGKIYTFGSYRLGVHH


    Database link: Q9NDU2

  • 常规说明

    Produced from outbred CD1 mice

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Constituents: 50% Glycerol
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 74 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性
    Following synthesis by RNA polymerase II, eukaryotic mRNA precursors (pre-mRNA) undergo a series of modifications, including capping at the 5' end, splicing and addition of a 3' poly(A) tail. The poly(A) tail has been shown to affect virtually all aspects of mRNA metabolism, including translation initiation and mRNA stabilization. In mammals, the synthesis of the poly(A) tail is catalyzed by poly(A) polymerase (PAP) with the help of the cleavage and polyadenylation specificity factor CPSF. Most PAPs are single polypeptide enzymes, and cDNA clones encoding them have been isolated from many different organisms. PAPs belong to a large superfamily of nucleotidyl transferases containing two signature features: a conserved catalytic domain adorned with three invariant carboxylates that are crucial for activity, and a helical turn motif involved in nucleotide binding.
  • 数据库链接
    • 别名
      • CG9854 antibody
      • hrg antibody
      • PAP antibody

    图片

    • All lanes : Anti-Poly A polymerase antibody (ab21867) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 74 kDa

    实验方案

    文献

    This product has been referenced in:
    • Soloveychik M  et al. Mitochondrial control through nutritionally regulated global histone H3 lysine-4 demethylation. Sci Rep 6:37942 (2016). WB . Read more (PubMed: 27897198) »

    See 1 Publication for this product

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