概述

  • 产品名称Anti-POLR3D抗体
    参阅全部 POLR3D 一抗
  • 描述
    兔多克隆抗体to POLR3D
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 175 and 225 of human POLR3D (NP_001713.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab86786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
IP Use at 10 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性POLR3D is a DNA dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • AI326118 antibody
    • AW489084 antibody
    • BN51 (BHK21) temperature sensitivity complementing antibody
    • BN51 antibody
    • BN51T antibody
    • DNA directed RNA polymerase III 47 kDa polypeptide antibody
    • DNA directed RNA polymerase III subunit D antibody
    • POLR3D antibody
    • Polymerase (RNA) III (DNA directed) polypeptide D antibody
    • Polymerase (RNA) III (DNA directed) polypeptide D, 44kDa antibody
    • Protein BN51 antibody
    • RNA polymerase III subunit C4 antibody
    • RPC4 antibody
    • RPC53 antibody
    • Temperature sensitive complementation cell cycle specific tsBN51 antibody
    • TSBN51 antibody
    see all

Anti-POLR3D antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling POLR3D with ab86786 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-POLR3D antibody (ab86786) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 44 kDa
    Observed band size : 44 kDa


    Exposure time : 3 minutes
  • Detection of POLR3D by Western Blot of Immunprecipitate.
    ab86786, at 1 µg/ml, staining POLR3D in HeLa whole cell lysate immunoprecipitated using ab86786 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-POLR3D antibody (ab86786)参考文献

ab86786 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab86786.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"