Recombinant fragment, corresponding to a region within N terminal amino acids 1-133 of Human POLR1D (AAH00889).
A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, HCT116 cell lysate, HBC435 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 15 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively.
Treacher Collins syndrome 2
Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.
Information by UniProt
DNA directed RNA polymerase I 16 kDa polypeptide antibody
DNA directed RNA polymerase I subunit D antibody
Western blot - POLR1D antibody (ab104115)
Anti-POLR1D antibody (ab104115) at 1/1000 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 15 kDa 15% SDS-PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - POLR1D antibody (ab104115)
ab104115, at 1/500 dilution, staining POLR1D in HBL435 xenograft by immunohistochemistry.
has not yet been referenced specifically in any publications.
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