重组Anti-POLDIP2抗体[EPR13832] - BSA and Azide free (ab232609)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13832] to POLDIP2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-POLDIP2抗体[EPR13832] - BSA and Azide free
参阅全部 POLDIP2 一抗 -
描述
兔单克隆抗体[EPR13832] to POLDIP2 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type HAP1 whole cell lysate. HeLa and HepG2 whole cell lysate.
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常规说明
ab232609 is the carrier-free version of ab181841.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13832 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232609于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
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IP |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. |
IP
Use at an assay dependent concentration. |
靶标
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序列相似性
Contains 1 apaG domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 26073 Human
- Entrez Gene: 67811 Mouse
- Entrez Gene: 287544 Rat
- Omim: 611519 Human
- SwissProt: Q9Y2S7 Human
- SwissProt: Q91VA6 Mouse
- Unigene: 241543 Human
- Unigene: 197493 Mouse
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别名
- 38 kDa DNA polymerase delta interaction protein antibody
- delta interacting protein 2 antibody
- DNA polymerase delta interaction protein p38 antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: POLDIP2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181841 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.ab181841 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when POLDIP2 knockout samples were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab181841 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLDIP2 with ab181841 at 1/350 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).
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Western blot analysis of HeLa cell lysate immunoprecipitated with ab181841 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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