概述

  • 产品名称
    Anti-POLDIP2抗体
    参阅全部 POLDIP2 一抗
  • 描述
    兔多克隆抗体to POLDIP2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human POLDIP2.

    (Peptide available as ab99870.)

  • 阳性对照
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab68663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

靶标

Anti-POLDIP2 antibody 图像



  • Predicted band size : 42 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: POLDIP2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab68663 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab68663 was shown to recognize POLDIP2 in wild type cells as signal was lost at the expected MW in POLDIP2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab68663 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-POLDIP2 antibody (ab68663) at 1 µg/ml + Liver (Human) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa


    Exposure time : 20 minutes

实验方案

Anti-POLDIP2 antibody (ab68663)参考文献

ab68663 has not yet been referenced specifically in any publications.

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