概述

  • 产品名称Anti-POLDIP1抗体
    参阅全部 POLDIP1 一抗
  • 描述
    兔多克隆抗体to POLDIP1
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide derived from a region between amino acids 26-75 of Human POLDIP1. Note: The exact sequence is proprietary.

  • 阳性对照
    • WB: Fetal heart lysate. IHC-P: Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab32974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1.5625e+006.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • 组织特异性Expressed in a wide variety of tissues.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the BACURD family.
    Contains 1 BTB (POZ) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BACD1_HUMAN antibody
    • BACURD1 antibody
    • BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1 antibody
    • BTB/POZ domain-containing protein KCTD13 antibody
    • FKSG86 antibody
    • hBACURD1 antibody
    • KCTD13 antibody
    • PDIP1 antibody
    • POLDIP 1 antibody
    • POLDIP1 antibody
    • Polymerase delta-interacting protein 1 antibody
    • Potassium channel tetramerisation domain containing 13 antibody
    • PP6832 antibody
    • TNFAIP1 like polymerase delta interacting protein 1 antibody
    • TNFAIP1-like protein antibody
    see all

Anti-POLDIP1 antibody 图像

  • Anti-POLDIP1 antibody (ab32974) at 0.25 µg/ml + fetal heart lysate

    Secondary
    HRP conjugated anti Rabbit IgG 1/50000 - 1/100000

    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling POLDIP1 with ab32974 at 4-8µg/ml. Cells with positive label (indicated by arrows) are neural cells. Magnification: 400X.

Anti-POLDIP1 antibody (ab32974)参考文献

This product has been referenced in:
  • Werwein E  et al. B-Myb promotes S-phase independently of its sequence-specific DNA binding activity and interacts with polymerase delta-interacting protein 1 (Pdip1). Cell Cycle 11:4047-58 (2012). Read more (PubMed: 23032261) »

See 1 Publication for this product

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