Anti-POLD3抗体(ab74307)

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概述

  • 产品名称
    Anti-POLD3抗体
    参阅全部 POLD3 一抗
  • 描述
    兔多克隆抗体to POLD3
  • 经测试应用
    适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residues 416 and 466 of human POLD3 (NP_006582.1)

  • 阳性对照
    • Lysate from HeLa or 293T cells

性能

应用

Our Abpromise guarantee covers the use of ab74307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
  • 应用说明
    Is unsuitable for IP.

    • 靶标

    • 功能
      Required for optimal DNA polymerase delta activity.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
      • 别名
        • DNA polymerase delta subunit 3 antibody
        • DNA polymerase delta subunit p66 antibody
        • DNA polymerase delta, subunit 3 antibody
        • DNA polymerase subunit delta p66 antibody
        • DPOD3_HUMAN antibody
        • KIAA0039 antibody
        • P66 antibody
        • P68 antibody
        • Pol delta C subunit (p66) antibody
        • Pold3 antibody
        • Polymerase (DNA directed), delta 3 antibody
        • Polymerase (DNA-directed), delta 3, accessory subunit antibody
        • PPP1R128 antibody
        • Protein phosphatase 1, regulatory subunit 128 antibody
        see all

      Anti-POLD3 antibody 图像

      • Western blot - POLD3 antibody (ab74307)
        Western blot - POLD3 antibody (ab74307)
        All lanes : Anti-POLD3 antibody (ab74307) at 0.1 µg/ml

        Lane 1 : Whole cell lysate from HeLa cells at 50 µg
        Lane 2 : Whole cell lysate from HeLa cells at 15 µg
        Lane 3 : Whole cell lysate from HeLa cells at 5 µg
        Lane 4 : Whole cell lysate from 293T cells at 50 µg


        Predicted band size : 51 kDa
        Observed band size : 63 kDa (why is the actual band size different from the predicted?)

      实验方案

      Anti-POLD3 antibody (ab74307)参考文献

      ab74307 has not yet been referenced specifically in any publications.

      发表研究结果有使用 ab74307？请让我们知道，以便我们可以引用本数据表中的参考文章。

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      1 Abreview or Q&A

      10
      Votes: Highest First

      Using a human cell lysate for western blotting; antibody is not working as expected; not detecting band at expected molecular weight

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab74307.

      To check the status of the order please contact o...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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