概述

  • 产品名称Anti-POGZ抗体
    参阅全部 POGZ 一抗
  • 描述
    兔多克隆抗体to POGZ
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: ADTDLFMECE EEELEPWQKI SDVIEDSVVE DYNSVDKTTT VSVSQQPVSA , corresponding to amino acids 2-51 of Human POGZ

  • 阳性对照
    • Brain lysate, human lung tissue and human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab66652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately >90 kDa (predicted molecular weight: 155 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.
  • 序列相似性Contains 9 C2H2-type zinc fingers.
    Contains 1 DDE domain.
    Contains 1 HTH CENPB-type DNA-binding domain.
  • 细胞定位Nucleus. Chromosome. Cytoplasm. According to some authors, it is not localized to mitotic chromatin (PubMed:19244240). Recruited to trimethylated 'Lys-9' of histone H3.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0461 antibody
    • MGC71543 antibody
    • Pogo transposable element with ZNF domain antibody
    • POGZ antibody
    • POGZ_HUMAN antibody
    • putative protein product of Nbla00003 antibody
    • SUHW5 antibody
    • suppressor of hairy wing homolog antibody
    • Suppressor of hairy wing homolog 5 antibody
    • Zinc finger protein 280E antibody
    • Zinc finger protein 635 antibody
    • ZNF280E antibody
    • ZNF635 antibody
    • ZNF635M antibody
    see all

Anti-POGZ antibody 图像

  • Anti-POGZ antibody (ab66652) at 5 µg/ml + Brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 155 kDa
    Observed band size : 45,>90 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling POGZ with ab66652 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analyss of human kidney tissue labelling POGZ with ab66652 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.

Anti-POGZ antibody (ab66652)参考文献

ab66652 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66652.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"