Anti-Pofut1抗体(ab74302)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to Pofut1
  • 特异性
    ab74302 detects endogenous levels of total Pofut1 protein.
  • 经测试应用
    适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from an internal region of human Pofat1.

  • 阳性对照
    • WB: Extracts from HeLa, Jurkat and HepG2 cells. IHC-P: Human lung carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100.

靶标

  • 功能
    Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.
  • 组织特异性
    Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 通路
    Protein modification; protein glycosylation.
  • 序列相似性
    Belongs to the glycosyltransferase 68 family.
  • 细胞定位
    Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FUT 12 antibody
    • FUT12 antibody
    • GDP fucose protein O fucosyltransferase 1 antibody
    • GDP-fucose protein O-fucosyltransferase 1 antibody
    • O Fuc T antibody
    • o fucosyltransferase protein antibody
    • O FucT 1 antibody
    • O FucT antibody
    • O FucT1 antibody
    • O FUT antibody
    • O-FucT-1 antibody
    • OFUT 1 antibody
    • OFUT antibody
    • OFUT1 antibody
    • OFUT1_HUMAN antibody
    • Peptide O fucosyltransferase1 antibody
    • Peptide-O-fucosyltransferase 1 antibody
    • Pofut 1 antibody
    • POFUT1 antibody
    • Protein O fucosyltransferase1 antibody
    see all

图片

  • ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.
  • All lanes : Anti-Pofut1 antibody (ab74302) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from HepG2 cells
    Lane 4 : extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 117 kDa,22 kDa,28 kDa,30 kDa. We are unsure as to the identity of these extra bands.

文献

ab74302 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Mar 22 2013

As for ab74302,I have received information that we do not have immunogen peptide available for sale in that case.

I apologize for any inconvenience.

Please do not hesitate to contact us if you need any more advice or information in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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