Anti-Pofut1抗体(ab74302)

概述

  • 产品名称Anti-Pofut1抗体
    参阅全部 Pofut1 一抗
  • 描述
    兔多克隆抗体to Pofut1
  • 特异性ab74302 detects endogenous levels of total Pofut1 protein.
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from an internal region of human Pofat1.

  • 阳性对照
    • WB: Extracts from HeLa, Jurkat and HepG2 cells. IHC-P: Human lung carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100.

靶标

  • 功能Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.
  • 组织特异性Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 通路Protein modification; protein glycosylation.
  • 序列相似性Belongs to the glycosyltransferase 68 family.
  • 细胞定位Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FUT 12 antibody
    • FUT12 antibody
    • GDP fucose protein O fucosyltransferase 1 antibody
    • GDP-fucose protein O-fucosyltransferase 1 antibody
    • O Fuc T antibody
    • o fucosyltransferase protein antibody
    • O FucT 1 antibody
    • O FucT antibody
    • O FucT1 antibody
    • O FUT antibody
    • O-FucT-1 antibody
    • OFUT 1 antibody
    • OFUT antibody
    • OFUT1 antibody
    • OFUT1_HUMAN antibody
    • Peptide O fucosyltransferase1 antibody
    • Peptide-O-fucosyltransferase 1 antibody
    • Pofut 1 antibody
    • POFUT1 antibody
    • Protein O fucosyltransferase1 antibody
    see all

Anti-Pofut1 antibody 图像

  • ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.
  • All lanes : Anti-Pofut1 antibody (ab74302) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from HepG2 cells
    Lane 4 : extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 117 kDa,22 kDa,28 kDa,30 kDa. We are unsure as to the identity of these extra bands.

Anti-Pofut1 antibody (ab74302)参考文献

ab74302 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 24°C
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Verified customer

提交于 Mar 22 2013

As for ab74302,I have received information that we do not have immunogen peptide available for sale in that case.

I apologize for any inconvenience.

Please do not hesitate to contact us if you need any more advice or information in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"