Anti-Podoplanin / gp36抗体(ab10274)

概述

  • 产品名称Anti-Podoplanin / gp36抗体
    参阅全部 Podoplanin / gp36 一抗
  • 描述
    兔多克隆抗体to Podoplanin / gp36
  • 经测试应用适用于: IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human).

性能

  • 形式Lyophilised:The lyophilized IgG is stable at 4°C for at least one month and for more than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six months at 2-4°C in the presence of a preservative. Reconstituted antibody can also be aliquoted and stored at –20°C to –70°C for at least 1 year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
WB
ICC/IF
  • 应用说明IF/ICC: 1/400.
    IP: 1/100.
    WB: 1/5000. Predicted molecular weight: 24 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
    • 组织特异性Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
    • 序列相似性Belongs to the podoplanin family.
    • 翻译后修饰Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
      The N-terminus is blocked.
    • 细胞定位Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Aggrus antibody
      • Glycoprotein 36 KD antibody
      • Glycoprotein 36 antibody
      • gp 36 antibody
      • GP 38 antibody
      • GP 40 antibody
      • gp36 antibody
      • GP38 antibody
      • GP40 antibody
      • HT1A 1 antibody
      • HT1A1 antibody
      • hT1alpha 1 antibody
      • hT1alpha 2 antibody
      • hT1alpha1 antibody
      • hT1alpha2 antibody
      • Lung type I cell membrane associated glycoprotein antibody
      • Lung type I cell membrane associated glycoprotein isoform a antibody
      • Lung type I cell membrane associated glycoprotein T1A 2 antibody
      • OTS 8 antibody
      • OTS8 antibody
      • OTTHUMP00000009640 antibody
      • OTTHUMP00000044504 antibody
      • PA2.26 antibody
      • PA2.26 antigen antibody
      • Pdpn antibody
      • PDPN_HUMAN antibody
      • Podoplanin antibody
      • PSEC0003 antibody
      • PSEC0025 antibody
      • T1 alpha antibody
      • T1 ALPHA GENE antibody
      • T1-alpha antibody
      • T1A 2 antibody
      • T1A antibody
      • TI1A antibody
      • TIA 2 antibody
      • TIA2 antibody
      see all

    Anti-Podoplanin / gp36 antibody (ab10274)参考文献

    This product has been referenced in:
    • Carden CP  et al. The association of PI3 kinase signaling and chemoresistance in advanced ovarian cancer. Mol Cancer Ther 11:1609-17 (2012). Read more (PubMed: 22556379) »
    • Kong BH  et al. Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens. Childs Nerv Syst : (2012). Read more (PubMed: 23143002) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (rat alveolar sections)
    Specification rat alveolar sections
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% saponin
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 08 2012

    Thank you for your inquiry.

    The antibody you mentioned is a polyclonal antibody against the full length protein. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope.

    I hope ...

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    Thank you very much for your enquiry. Ab10274 has yet to be tested on formalin fixed, paraffin embedded tissue so I can only refer you to our general protocols which can be found on our homepage. I would suggest starting with the heat mediated pressure...

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    Thank you for your enquiry and your patience. We are very sorry to hear that you are having problem with this antibody We would like to confirm that this antibody has been tested for IF application. We would suggest the followings: 1. Test the d...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"