概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to PNR
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Recombinant fusion protein (His tagged):

    LSDGDSTSDQQSTRDYPHFS

    , corresponding to amino acids 5-24 of Fruit fly (Drosophila melanogaster) PNR

  • 常规说明

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 38 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能
    Orphan receptor. Ligands are likely small molecules, either sharing some similarities with trace amine as, e.g. derivatives of indolamines (such as 5-methoxytryptamine) or of phenylethylamines (such as phenylethanolamine) or being any kind of metabolite of amino acids or biogenic amine neurotransmitters.
  • 组织特异性
    Expressed almost exclusively in skeletal muscle and selected areas of the brain, such amygdala, hippocampus, caudate nucleus, thalamus and hypothalamus. Weak expression is also find in substantia nigra.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 别名
    • dGATA A antibody
    • GATA binding factor A antibody
    • Photoreceptor specific nuclear receptor fragment antibody
    • PNR antibody
    • Putative neurotransmitter receptor antibody
    • Taar5 antibody
    • TAAR5_HUMAN antibody
    • TaR-5 antibody
    • Trace amine associated receptor 5 antibody
    • Trace amine receptor 5 antibody
    • Trace amine-associated receptor 5 antibody
    • Transcription factor GATA A antibody
    see all

图片

  • All lanes : Anti-PNR antibody (ab52782) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a Tagged irrelevant antigen (Negative control)
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (Tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase at 1/5000 dilution

    Predicted band size : 38 kDa

实验方案

文献

ab52782 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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