兔多克隆抗体to PNMT - Carboxyterminal end
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Protein A purified
This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/100. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).
Converts noradrenaline to adrenaline.
Catecholamine biosynthesis; (R)-adrenaline biosynthesis; (R)-adrenaline from (R)-noradrenaline: step 1/1.
Belongs to the NNMT/PNMT/TEMT family.
Information by UniProt
- Noradrenaline N-methyltransferase antibody
- PENT antibody
- Phenylethanolamine N-methyltransferase antibody
Western blot - PNMT antibody (ab71096)
Anti-PNMT antibody - Carboxyterminal end (ab71096) at 1/60 dilution + T47D cell lysate at 35 µgPredicted band size :
31 kDaObserved band size :
28 kDa (why is the actual band size different from the predicted?
)Additional bands at :
55 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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