Anti-PNMT抗体[1D2] (ab119784)

概述

  • 产品名称Anti-PNMT抗体[1D2]
    参阅全部 PNMT 一抗
  • 描述
    小鼠单克隆抗体[1D2] to PNMT
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Monkey
  • 免疫原

    Recombinant full length Human PNMT produced in HEK293T cells (NP_002677).

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY PNMT cDNA; HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cell extracts; Human endometrium, endometrium adenocarcinoma and bladder carcinoma tissues; COS7 cells transiently transfected with pCMV6-ENTRY PNMT.
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

应用

Our Abpromise guarantee covers the use of ab119784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 31 kDa.
IHC-P 1/150.
ICC/IF 1/100.

靶标

Anti-PNMT antibody [1D2] 图像

  • All lanes : Anti-PNMT antibody [1D2] (ab119784) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PNMT cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 31 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206586)
  • All lanes : Anti-PNMT antibody [1D2] (ab119784) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 31 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206586)
  • ab119784, at 1/100 dilution, staining PNMT in COS7 cells transiently transfected with pCMV6-ENTRY PNMT cDNA by Immunofluorescence.
  • ab119784, at 1/150 dilution, staining PNMT in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab119784, at 1/150 dilution, staining PNMT in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab119784, at 1/150 dilution, staining PNMT in paraffin-embedded Human endometrium tissue by Immunohistochemistry.

Anti-PNMT antibody [1D2] (ab119784)参考文献

ab119784 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - Triton x-100, 0.01%
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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提交于 Jun 17 2015

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I have talked to our supplier of this antibody and they have told me that the higher MW protein could be due to dimerization caused MW abnormality. However...

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Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Est...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"