概述

  • 产品名称Anti-PNK抗体
    参阅全部 PNK 一抗
  • 描述
    兔多克隆抗体to PNK
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 250 and 300 of human PNK (TrEMBL entry Q96T60)

  • 阳性对照
    • HeLa whole cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2500 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
    • 组织特异性Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
    • 疾病相关Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
    • 序列相似性In the N-terminal section; belongs to the DNA 3' phosphatase family.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2''(3'')-polynucleotidase antibody
      • 2'(3')-polynucleotidase antibody
      • Bifunctional polynucleotide phosphatase/kinase antibody
      • DEM 1 antibody
      • DEM1 antibody
      • DNA 5' kinase/3' phosphatase antibody
      • DNA 5''-kinase/3''-phosphatase antibody
      • EIEE10 antibody
      • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
      • MCSZ antibody
      • PNK 1 antibody
      • PNK1 antibody
      • Pnkp antibody
      • PNKP DNA kinase antibody
      • PNKP_HUMAN antibody
      • Polynucleotide 3'-phosphatase antibody
      • Polynucleotide 5' hydroxyl kinase antibody
      • Polynucleotide 5''-hydroxyl-kinase antibody
      • Polynucleotide kinase 3 prime phosphatase antibody
      • Polynucleotide kinase 3' phosphatase antibody
      • Polynucleotide Kinase antibody
      • Polynucleotide kinase-3''-phosphatase antibody
      see all

    Anti-PNK antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PNK with ab70739 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-PNK antibody (ab70739) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg


      Predicted band size : 57 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa,130 kDa,160 kDa,55 kDa,<50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 seconds

    Anti-PNK antibody (ab70739)参考文献

    ab70739 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Hep G2)
    Loading amount 100000 cells
    Specification Hep G2
    Treatment 15 nM siRNA transfection for 72 hours
    Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Shijian Zhang

    Verified customer

    提交于 Aug 03 2011

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A549 tumour cells)
    Loading amount 50 µg
    Specification A549 tumour cells
    Treatment PNK siRNA treated
    Gel Running Conditions Reduced Denaturing (8% tris glycine)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Mr. Paul Eve

    Verified customer

    提交于 Jun 16 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"