Anti-Pneumolysin抗体[PLY-4] (ab71810)

概述

  • 产品名称
    Anti-Pneumolysin抗体[PLY-4]
    参阅全部 Pneumolysin 一抗
  • 描述
    小鼠单克隆抗体[PLY-4] to Pneumolysin
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ICC/IF, Neutralisingmore details
  • 免疫原

    Recombinant full length protein

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 50mM Tris buffered saline, pH 7.5
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    PLY-4
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 29636428
Neutralising Use at an assay dependent concentration. PubMed: 8550195

靶标

  • 细胞定位
    Plasma membrane

图片

  • Coomassie Blue-stained gel (lane 1) and Western blots of purified recombinant PLY, 3 µg per lane, with mouse monoclonal antibodies PLY-4 (lane 2), PLY-5 (lane 3) and PLY-7 (lane 4); a PLY-irrelevant mouse monoclonal antibody 1.4G8.66 served as control (lane 5).

实验方案

文献

This product has been referenced in:
  • Codemo M  et al. Immunomodulatory Effects of Pneumococcal Extracellular Vesicles on Cellular and Humoral Host Defenses. MBio 9:N/A (2018). WB, ICC/IF . Read more (PubMed: 29636428) »
  • Shenoy AT  et al. Streptococcus pneumoniae in the heart subvert the host response through biofilm-mediated resident macrophage killing. PLoS Pathog 13:e1006582 (2017). Read more (PubMed: 28841717) »

See all 9 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Streptococcus pneumoniae Cell lysate - whole cell (Streptococcus pneumoniae: serotype 3, strain A66)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Streptococcus pneumoniae: serotype 3, strain A66
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2017

Gracias por habernos contactado.
Le confirmo la información que me proporcionan mis compañeros del laboratorio: para inmunizar los ratones con los que se produjo ab71810 se utilizó PLY entera.
No dudes en contactarnos para cualquier otra cons...

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Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending two free of charge vials of ab71810 on the order ***, which should arrive tomorrow. I've requested that these vials be in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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