概述

  • 产品名称Anti-PMP70抗体(Biotin)
    参阅全部 PMP70 一抗
  • 描述
    兔多克隆抗体to PMP70 (Biotin)
  • 偶联物Biotin
  • 特异性ab28499 recognises Peroxisomal membrane protein 70 (PMP70).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken
  • 免疫原

    Synthetic peptide:

    NYEFKKITEDTVEFGS

    , corresponding to C terminal amino acids 644 - 659 of Rat PMP70

  • 阳性对照
    • Mouse kidney protein extract

性能

应用

Our Abpromise guarantee covers the use of ab28499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 74 kDa.

靶标

  • 功能Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
  • 序列相似性Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • 细胞定位Peroxisome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 70 kDa peroxisomal membrane protein antibody
    • ABC 43 antibody
    • ABC D3 antibody
    • ABC43 antibody
    • ABCD 3 antibody
    • Abcd3 antibody
    • ABCD3 protein antibody
    • ABCD3_HUMAN antibody
    • ATP binding cassette sub family D (ALD) member 3 antibody
    • ATP binding cassette sub family D member 3 antibody
    • ATP-binding cassette sub-family D member 3 antibody
    • Peroxisomal membrane protein 1 antibody
    • PMP 70 antibody
    • PMP70 antibody
    • PXMP 1 antibody
    • PXMP1 antibody
    • ZWS2 antibody
    see all

Anti-PMP70 antibody (Biotin) 图像

  • All lanes : Anti-PMP70 antibody (Biotin) (ab28499) at 1/1000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit

    Predicted band size : 74 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent analysis of PMP70 staining in A459 cells with ab28499.

  • Immunofluorescent analysis of PMP70 staining in NS-1 cells with ab28499.

  • Immunofluorescent analysis of PMP70 staining in HMVEC cells with ab28499.

  • Immunofluorescent analysis of PMP70 staining in p19 cells with ab28499.

  • Flow cytometry analysis of PMP70 in HepG2 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab28499 at a dilution of 0.5 μg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight® 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow cytometry analysis of PMP70 in 293T cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab28499 at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight® 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Anti-PMP70 antibody (Biotin) (ab28499)参考文献

This product has been referenced in:
  • Falcon A  et al. FATP2 is a hepatic fatty acid transporter and peroxisomal very long-chain acyl-CoA synthetase. Am J Physiol Endocrinol Metab 299:E384-93 (2010). Purification ; Mouse . Read more (PubMed: 20530735) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab28499.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"