概述

  • 产品名称Anti-PMP70抗体
    参阅全部 PMP70 一抗
  • 描述
    兔多克隆抗体to PMP70
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Rat PMP70.

    (Peptide available as ab94821.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Rat liver; Rat kidney; Human liver; Mouse Lung; as well as the following whole cell lysates: HepG2; HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab85550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 75 kDa).

靶标

Anti-PMP70 antibody 图像

  • All lanes : Anti-PMP70 antibody (ab85550) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Kidney (Rat) Tissue Lysate
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,28 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab85550 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85550, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml.
  • IHC image of Ab85550 staining in Human Cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab85550, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-PMP70 antibody (ab85550) at 1 µg/ml

    Lane 1 : Lung (Mouse) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

Anti-PMP70 antibody (ab85550)参考文献

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