Anti-PMP70抗体(ab81904)

概述

  • 产品名称Anti-PMP70抗体
    参阅全部 PMP70 一抗
  • 描述
    山羊多克隆抗体to PMP70
  • 特异性ab81904 is expected to recognize one isoform (NP_002849.1) only.
  • 经测试应用适用于: WB, ELISA, ICCmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Orangutan
  • 免疫原

    Synthetic peptide:

    C-PDGREDQKRKGISD

    , corresponding to internal sequence amino acids 526-539 of Human PMP70 (NP_002849.1).

  • 阳性对照
    • Human Kidney lysate.

性能

应用

Our Abpromise guarantee covers the use of ab81904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).
ELISA 1/16000. Peptide ELISA
ICC Use a concentration of 2.5 - 3.8 µg/ml.

靶标

Anti-PMP70 antibody 图像

  • Anti-PMP70 antibody (ab81904) at 0.2 µg/ml + Human Kidney Lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 75 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Anti-PMP70 antibody (ab81904) at 1/10000 dilution (in Licor Blocking Buffer for 2 hours at 25°C) + Nuclear lysate of Hela cells at 10 µg

    Secondary
    An Alexa Fluor®680-conjugated Donkey anti-goat IgG polyclonal at 1/10000 dilution

    Predicted band size : 75 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab81904 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-PMP70 antibody (ab81904)参考文献

ab81904 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Hela cells)
Loading amount 10 µg
Specification Hela cells
Gel Running Conditions Reduced Denaturing (4-12% Gradient Gel)
Blocking step Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Jun 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"