Anti-PML蛋白抗体,prediluted (ab31102)

概述

  • 产品名称
    Anti-PML蛋白抗体,prediluted
    参阅全部 PML Protein 一抗
  • 描述
    兔多克隆抗体to PML蛋白,prediluted
  • 宿主
    Rabbit
  • 特异性
    ab31102 recognsies PML protein.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    PSPSPTERAPASEEE

    , corresponding to amino acids 37-51 of Human PML Protein

  • 阳性对照
    • K562 cells. Lymph node tissue.

性能

应用

Our Abpromise guarantee covers the use of ab31102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明
    IHC-P: ready to use for 30 minutes at RT. Requires boiling of tissue sections in 10mM citrate buffer pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Key component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post-translational modification of target proteins, promoting protein-protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4. Inhibits specifically the activity of the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.
    • 疾病相关
      Note=A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
    • 序列相似性
      Contains 2 B box-type zinc fingers.
      Contains 1 RING-type zinc finger.
    • 结构域
      Interacts with PKM2 via its coiled-coil domain.
      Binds arsenic via the RING-type zinc finger.
    • 翻译后修饰
      Ubiquitinated; mediated by RNF4, SIAH1 or SIAH2 and leading to subsequent proteasomal degradation. 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4 is polysumoylation-dependent.
      Undergoes 'Lys-11'-linked sumoylation. Sumoylation on all three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The PML-RARA fusion protein requires the coiled-coil domain for sumoylation. Desumoylated by SENP2 and SENP6. Arsenic induces PML and PML-RARA oncogenic fusion proteins polysumoylation and their subsequent RNF4-dependent ubiquitination and proteasomal degradation, and is used as treatment in acute promyelocytic leukemia (APL).
      Phosphorylated in response to DNA damage, probably by ATR.
      Acetylation may promote sumoylation and enhance induction of apoptosis.
    • 细胞定位
      Nucleus > nucleoplasm. Cytoplasm. Nucleus > PML body. Nucleus > nucleolus. Endoplasmic reticulum membrane. Early endosome membrane. Sumoylated forms localize to the PML nuclear bodies. The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclear localization signal are cytoplasmic. Detected in the nucleolus after DNA damage. Sequestered in the cytoplasm by interaction with rabies virus phosphoprotein.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Acure promyelocytic leukemia, inducer of antibody
      • MYL antibody
      • Pml antibody
      • PML_HUMAN antibody
      • PP8675 antibody
      • Probable transcription factor PML antibody
      • Promyelocytic leukemia antibody
      • Promyelocytic leukemia inducer of antibody
      • Promyelocytic leukemia protein antibody
      • Protein PML antibody
      • RING finger protein 71 antibody
      • RNF 71 antibody
      • RNF71 antibody
      • TRIM 19 antibody
      • Tripartite motif protein TRIM19 antibody
      • Tripartite motif-containing protein 19 antibody
      see all

    图片

    • ab31102 staining human PML in human lymph node tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

    文献

    ab31102 has not yet been referenced specifically in any publications.

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