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Our Abpromise guarantee covers the use of ab3528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/300. PubMed: 16803870|
|ELISA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IP||Use a concentration of 1 µg/ml.|
|WB||1/1000. This antibody detects ~134 kDa and ~130 kDa proteins representing PMCA 1a and 1b ATPase from rat samples.|
ab3528 at 1/100 staining rat brain hippocacmpal slices and slice cultures by IHC-P. The tissue was paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with BSA before incubation with the antibody for 12 hours. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr R Empson