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Raised against yeast membrane preps, screened by immunofluorescence and blotting.
Our Abpromise guarantee covers the use of ab4645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Predicted molecular weight: 100 kDa. (cell lysates, ECL detection). For non-ECL western detection methods, 1/1000 - 1/5000. Also detects Pma1p-containing fusion proteins on western blots.|
|ICC/IF||Use at an assay dependent dilution.|