The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human Plzf peptide (ab39353).
功能Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
组织特异性Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.
通路Protein modification; protein ubiquitination.
疾病相关Skeletal defects, genital hypoplasia, and mental retardation A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA.
序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 1 BTB (POZ) domain. Contains 9 C2H2-type zinc fingers.
Kirsch S et al. The (pro)renin receptor mediates constitutive PLZF-independent pro-proliferative effects which are inhibited by bafilomycin but not genistein. Int J Mol Med33:795-808 (2014).
Read more (PubMed: 24424509) »
Jiao B et al. 8-CPT-cAMP/all-trans retinoic acid targets t(11;17) acute promyelocytic leukemia through enhanced cell differentiation and PLZF/RARa degradation. Proc Natl Acad Sci U S A110:3495-500 (2013).
Read more (PubMed: 23382200) »