概述

  • 产品名称Anti-Plzf抗体[5B3]
    参阅全部 Plzf 一抗
  • 描述
    小鼠单克隆抗体[5B3] to Plzf
  • 经测试应用适用于: ELISA, WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant fragment of Human Plzf expressed in E. Coli.

  • 阳性对照
    • Hela cell lysate. Hela cells

性能

应用

Our Abpromise guarantee covers the use of ab104854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 74 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • 组织特异性Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.
  • 通路Protein modification; protein ubiquitination.
  • 疾病相关Skeletal defects, genital hypoplasia, and mental retardation
    A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 9 C2H2-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI467657 antibody
    • lu antibody
    • PLZF antibody
    • Promyelocytic leukemia zinc finger protein antibody
    • ZBT16_HUMAN antibody
    • ZBTB16 antibody
    • Zfp145 antibody
    • Zinc finger and BTB domain containing 16 antibody
    • zinc finger and BTB domain containing protein 16 antibody
    • Zinc finger and BTB domain-containing protein 16 antibody
    • Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) antibody
    • Zinc finger protein 145 antibody
    • Zinc finger protein PLZF antibody
    • Znf145 antibody
    see all

Anti-Plzf antibody [5B3] 图像

  • Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate

    Predicted band size : 74 kDa
  • Immunofluorescence analysis of Hela cells using ab104854 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Plzf antibody [5B3] (ab104854)参考文献

This product has been referenced in:
  • Yu-Ching Lin Z  et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Marmoset (common) . Read more (PubMed: 25624265) »
  • Schwab KR  et al. Arrested spermatogenesis and evidence for DNA damage in PTIP mutant testes. Dev Biol 373:64-71 (2013). Read more (PubMed: 23063797) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Monodelphis domestica Cell (testis)
Permeabilization Yes - 0.5% Triton X-100
Specification testis
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde
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提交于 Sep 14 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult testis)
Specification Adult testis
Permeabilization No
Fixative Formaldehyde
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提交于 Jun 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"