Anti-Plzf抗体[5B3] (ab104854)
Key features and details
- Mouse monoclonal [5B3] to Plzf
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Plzf抗体[5B3]
参阅全部 Plzf 一抗 -
描述
小鼠单克隆抗体[5B3] to Plzf -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Purified recombinant fragment of Human Plzf expressed in E. Coli.
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阳性对照
- HeLa whole cell lysate (ab150035). HeLa cells
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常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR161367-22 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
5B3 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104854于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 74 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 74 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
组织特异性
Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Skeletal defects, genital hypoplasia, and mental retardation
A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 9 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7704 Human
- Omim: 176797 Human
- SwissProt: Q05516 Human
- Unigene: 591945 Human
- Unigene: 682144 Human
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别名
- AI467657 antibody
- lu antibody
- PLZF antibody
see all
图片
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Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate
Predicted band size: 74 kDa -
Immunofluorescence analysis of Hela cells using ab104854 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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Datasheet download
文献 (15)
ab104854 被引用在 15 文献中.
- Bashiri Z et al. Proliferation of human spermatogonial stem cells on optimized PCL/Gelatin nanofibrous scaffolds. Andrologia 54:e14380 (2022). PubMed: 35083770
- Liu R et al. SETDB1 Regulates Porcine Spermatogonial Adhesion and Proliferation through Modulating MMP3/10 Transcription. Cells 11:N/A (2022). PubMed: 35159180
- Mao GP et al. Characterization, isolation, and culture of spermatogonial stem cells in Macaca fascicularis. Asian J Androl 23:240-248 (2021). PubMed: 33533740
- Xu H et al. Derivation and propagation of spermatogonial stem cells from human pluripotent cells. Stem Cell Res Ther 11:408 (2020). PubMed: 32967715
- Zheng Y et al. Establishment of cell lines with porcine spermatogonial stem cell properties. J Anim Sci Biotechnol 11:33 (2020). PubMed: 32308978