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Abcam's PLTP Activity Fluorometric Assay Kit uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of PLTP. PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Excitation: 465 nm; Emission: 535 nm).
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This product is being discontinued. We have created an improved version of this product: PLTP Activity Assay Kit II (Fluorometric) (ab196998).
Plasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of high-density lipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activity is related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity, and insulin resistance.
|PLTP Acceptor Molecule||Blue||1 x 1ml|
|PLTP Assay Buffer (10X)||Clear||1 x 5ml|
|PLTP Donor Molecule||Violet||1 x 1ml|
|Positive Control (Rabbit Serum)||1 x 30µl|
Our Abpromise guarantee covers the use of ab65386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab65386 has not yet been referenced specifically in any publications.