概述

  • 产品名称Anti-PLS1抗体
    参阅全部 PLS1 一抗
  • 描述
    小鼠单克隆抗体to PLS1
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Partial recombinant human PLS1 protein (NP_002661: amino acids 1 to 103) with a 26 kDa tag

  • 阳性对照
    • Recombinant PLS1 protein; Jurkat cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab76510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at a concentration of 0.1 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight for the full length protein: 70 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. Fimbrin is a 68-kD actin-bundling protein.
    • 细胞定位Cytoplasm
    • 数据库链接
    • 别名
      • Fimbrin antibody
      • I Fimbrin antibody
      • I Plastin antibody
      • Intestine specific plastin antibody
      • Plastin 1 (I isoform) antibody
      • Plastin 1 antibody
      • Plastin1 antibody
      see all

    Anti-PLS1 antibody 图像

    • Anti-PLS1 antibody (ab76510) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 70 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-PLS1 antibody (ab76510) at 5 µg/ml + Jurkat cell lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 70 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • Application of ab76510 in Elisa.
      Detection limit for recombinant, tagged PLS1 is approximately 3ng/ml as a capture antibody.

    Anti-PLS1 antibody (ab76510)参考文献

    ab76510 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"