PLK4 overexpression 293T裂解液(全细胞) (ab94256)

概述

  • 产品名称PLK4 overexpression 293T裂解液(全细胞)
  • 描述
    PLK4 overexpression裂解液
  • 常规说明ab94256 is a 293T cell transfected lysate in which Human PLK4 has been transiently over-expressed using a pCMV-PLK4 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Contains 1 POLO box domain. Contains 1 protein kinase domain. PTM: Ubiquitinated; leading to its degradation by the proteasome. Tyrosine-phosphorylated by TEC.

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应用

Our Abpromise guarantee covers the use of ab94256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

PLK4 overexpression 293T lysate (whole cell) 图像

  • ab94256 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-PLK4 antibody (ab56752) at 1/500 dilution

    Lane 1 : PLK4 overexpression 293T lysate (whole cell) (ab94256)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

PLK4 overexpression 293T lysate (whole cell) (ab94256)参考文献

ab94256 has not yet been referenced specifically in any publications.

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