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Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human PLK4.
(Peptide available as ab71393.)
Our Abpromise guarantee covers the use of ab71394 in the following tested applications.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 109 kDa).|
ICC/IF image of ab71394 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71394, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The 90 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to PLK4.
This image is courtesy of an Abreview submitted by Antibody Solutions