- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-PLK3抗体
参阅全部 PLK3 一抗 -
描述小鼠多克隆抗体to PLK3
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宿主Mouse
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经测试应用适用于: WBmore details
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种属反应性与反应: Human, Recombinant fragment
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免疫原
PLK3 (NP_004064.2, 1 a.a. ~ 646 a.a) full-length human protein.
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阳性对照
- PLK3 transfected 293T cell lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
纯度Protein G purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab67922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 72 kDa. This antibody has been tested on the recombinant fragment only and we cannot guarantee that it will work on the endogenous protein |
靶标
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功能Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.
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组织特异性Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
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序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain. -
翻译后修饰Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 1263 Human
- Omim: 602913 Human
- SwissProt: Q9H4B4 Human
- Unigene: 632415 Human
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别名
- CNK antibody
- Cytokine Inducible Kinase antibody
- Cytokine-inducible serine/threonine-protein kinase antibody
see all
图片
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Western blot - Anti-PLK3 antibody (ab67922)This image is courtesy of an Abreview submitted by Armen PetrosyanAnti-PLK3 antibody (ab67922) at 1/2000 dilution + HeLa cell lysate at 18 µg
Secondary
HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 95 kDa (why is the actual band size different from the predicted?)
Exposure time: 45 seconds
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Western blot - Anti-PLK3 antibody (ab67922)All lanes : Anti-PLK3 antibody (ab67922) at 1/500 dilution
Lane 1 : PLK3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 80 kDa (why is the actual band size different from the predicted?)
文献
This product has been referenced in:
- Petrosyan A et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). WB ; Human . Read more (PubMed: 25086069) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"