Customer reviews and FAQs

概述

  • 产品名称Anti-PLK3抗体
    参阅全部 PLK3 一抗
  • 描述
    小鼠多克隆抗体to PLK3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human, Recombinant fragment
  • 免疫原

    PLK3 (NP_004064.2, 1 a.a. ~ 646 a.a) full-length human protein.

  • 阳性对照
    • PLK3 transfected 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 72 kDa. This antibody has been tested on the recombinant fragment only and we cannot guarantee that it will work on the endogenous protein

靶标

  • 功能Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.
  • 组织特异性Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CNK antibody
      • Cytokine Inducible Kinase antibody
      • Cytokine-inducible serine/threonine-protein kinase antibody
      • FGF inducible kinase antibody
      • FGF-inducible kinase antibody
      • FNK antibody
      • PLK-3 antibody
      • Plk3 antibody
      • PLK3_HUMAN antibody
      • Polo Like Kinase 3 antibody
      • Polo-like kinase 3 antibody
      • PRK antibody
      • Proliferation Related Kinase antibody
      • Proliferation-related kinase antibody
      • Serine/threonine-protein kinase PLK3 antibody
      see all

    Anti-PLK3 antibody 图像

    • Western blot - Anti-PLK3 antibody (ab67922)
      Western blot - Anti-PLK3 antibody (ab67922)This image is courtesy of an Abreview submitted by Armen Petrosyan
      Anti-PLK3 antibody (ab67922) at 1/2000 dilution + HeLa cell lysate at 18 µg

      Secondary
      HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 72 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)


      Exposure time : 45 seconds

      This image is courtesy of an Abreview submitted by Armen Petrosyan

      See Abreview

    • Western blot - PLK3 antibody (ab67922)
      Western blot - PLK3 antibody (ab67922)
      All lanes : Anti-PLK3 antibody (ab67922) at 1/500 dilution

      Lane 1 : PLK3 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Anti-PLK3 antibody (ab67922)参考文献

    This product has been referenced in:
    • Petrosyan A  et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). WB ; Human . Read more (PubMed: 25086069) »

    See 1 Publication for this product

    发表研究结果有使用 ab67922？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    2 Abreviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PLK3 antibody

     Good
    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Panc-1 pancreartic cancer cells)
    Permeabilization Yes - 0.2% Triton X-100
    Specification Panc-1 pancreartic cancer cells
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Nov 19 2015

    Western blot abreview for Anti-PLK3 antibody

     Excellent
    Abreviews
    Application Western blot
    Loading amount 18 µg
    Gel Running Conditions Reduced Denaturing (4-15% gradient SDS-PAGE)
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    abreview image
    Username

    Dr. Armen Petrosyan

    Verified customer

    提交于 Nov 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"