Anti-PLK1 (phospho T210)抗体(ab12157)


  • 产品名称
    Anti-PLK1 (phospho T210)抗体
    参阅全部 PLK1 一抗
  • 描述
    兔多克隆抗体to PLK1 (phospho T210)
  • 宿主
  • 特异性
    Detects a band at 55kDa in mouse A20 cells that we believe corresponds to Plk1 (predicted molecular weight 68kDa). Also gives positive staining in immunohistochemistry in human colon carcinoma as expected. However, we have been unable to conclusively demonstrate the specificity of the antibody for phospho T210 Plk1.
  • 经测试应用
    适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Xenopus laevis, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide: C-GERKKTPLCGT, corresponding to amino acids 205-214 of Human Plk1.



Our Abpromise guarantee covers the use of ab12157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/2000. Predicted molecular weight: 68 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/3000 - 1/12000.
ICC/IF Use at an assay dependent concentration.


  • 功能
    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • 组织特异性
    Placenta and colon.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • 发展阶段
    Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • 翻译后修饰
    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • 细胞定位
    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell cycle regulated protein kinase antibody
    • PLK 1 antibody
    • PLK antibody
    • PLK-1 antibody
    • plk1 antibody
    • PLK1_HUMAN antibody
    • Polo like kinase 1 antibody
    • Polo-like kinase 1 antibody
    • Serine/threonine protein kinase 13 antibody
    • Serine/threonine protein kinase PLK1 antibody
    • Serine/threonine-protein kinase 13 antibody
    • Serine/threonine-protein kinase PLK1 antibody
    • STPK 13 antibody
    • STPK13 antibody
    see all


  • Anti-PLK1 (phospho T210) antibody (ab12157) at 1/500 dilution + Mouse A20 cell lysate

    Predicted band size: 68 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 23 kDa, 40 kDa, 42 kDa. We are unsure as to the identity of these extra bands.

    We believe the 55kDa band represents Plk1. However, we cannot be sure that the antibody is detecting phospho T210 Plk1 specifically.
  • ab12157 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded. Detection with AEC (pink), nuclear counterstaining with Haematoxylin (blue).
  • ab12157 (1/200) detecting Plk1 (phospho T210) in formalin-fixed paraffin embedded human breast carcinoma tissue.


This product has been referenced in:
  • Oshima K  et al. Mutational landscape, clonal evolution patterns, and role of RAS mutations in relapsed acute lymphoblastic leukemia. Proc Natl Acad Sci U S A 113:11306-11311 (2016). Read more (PubMed: 27655895) »
  • Li R  et al. Involvement of polo-like kinase 1 (Plk1) in mitotic arrest by inhibition of the mitogen-activated protein kinase--extracellular signal-regulated kinase--Ribosomal S6 kinase 1 (MEK-ERK-RSK1) cascade. J Biol Chem : (2012). WB . Read more (PubMed: 22427657) »

See all 3 Publications for this product


Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryo)
0-4hr embryo
control shRNA and wee shRNA embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Richelle Sopko

Verified customer

提交于 Aug 02 2013

Thank you for contacting Abcam.

The immunogen used for this product has 100% homology with the mouse protein ( This product has not yet been tested in this species however. Because this has been raised ...

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