- 数据表
- 具体的参考文献 (13)
- 实验方案
概述
-
产品名称Anti-PLK1 (phospho T210)抗体[2A3]
参阅全部 PLK1 一抗 -
描述小鼠单克隆抗体[2A3] to PLK1 (phospho T210)
-
宿主Mouse
-
特异性ab39068 recognises Plk1 (phospho T210).
-
经测试应用适用于: ICC/IF, Flow Cyt, WBmore details
-
种属反应性与反应: Chicken, Human
-
免疫原
Synthetic peptide corresponding to Human PLK1.
-
阳性对照
- Extracts from overnight nocodazole treated Hela cells.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
存储溶液Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
纯度Protein G purified
-
纯化说明ab39068 is an affinity purified IgG.
-
克隆单克隆
-
克隆编号2A3
-
同种型IgG1
-
轻链类型kappa
-
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
应用
Our Abpromise guarantee covers the use of ab39068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/300. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. |
靶标
-
功能Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
-
组织特异性Placenta and colon.
-
序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain. -
发展阶段Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
-
翻译后修饰Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint. -
细胞定位Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
- Information by UniProt
-
数据库链接
- Entrez Gene: 5347 Human
- Omim: 602098 Human
- SwissProt: P53350 Human
- Unigene: 592049 Human
-
别名
- Cell cycle regulated protein kinase antibody
- PLK 1 antibody
- PLK antibody
see all
图片
-
![Western blot - Plk1 (phospho T210) antibody [2A3] (ab39068)](http://www.abcam.cn/ps/datasheet/Images/39/ab39068/WB Plk1 phospho.jpg)
Western blot - Plk1 (phospho T210) antibody [2A3] (ab39068)All lanes : Anti-PLK1 (phospho T210) antibody [2A3] (ab39068) at 1 µg/ml
Lane 1 : Extracts from untreated Hela cells
Lane 2 : Extracts from overnight nocodazole treated Hela cells
Predicted band size: 68 kDa
Observed band size: 66 kDa (why is the actual band size different from the predicted?) -
![Immunocytochemistry/ Immunofluorescence - PLK1 (phospho T210) antibody [2A3] (ab39068)](http://www.abcam.cn/ps/datasheet/Images/39/ab39068/ab39068_review_12338.jpg)
Immunocytochemistry/ Immunofluorescence - PLK1 (phospho T210) antibody [2A3] (ab39068)This image is courtesy of an anonymous Abreviewab39068 staining chicken B lymphoma cells in prometaphase by ICC/IF. Cells were PFA fixed and permeabilized in 0.15% Triton X-100 prior to blocking in 1% BSA for 1 hour at 4°C. The primary antibody was diluted 1/300 (PBS/1%BSA) and incubated with the sample for 1 hour at 37°C. A Texas Red® conjugated goat polyclonal to mouse, diluted 1/200, was used as the secondary.
实验方案
文献
This product has been referenced in:
- Dumitru AMG et al. Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability. Elife 6:N/A (2017). Read more (PubMed: 29154753) »
- Naylor RM et al. Nuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidy. J Clin Invest 126:543-59 (2016). Read more (PubMed: 26731471) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"