Anti-PLK1 (phospho T210)抗体[2A3] (ab39068)


  • 产品名称Anti-PLK1 (phospho T210)抗体[2A3]
    参阅全部 PLK1 一抗
  • 描述
    小鼠单克隆抗体[2A3] to PLK1 (phospho T210)
  • 特异性ab39068 recognises Plk1 (phospho T210).
  • 经测试应用适用于: ICC/IF, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Chicken, Human
  • 免疫原

    Synthetic peptide corresponding to Human PLK1.

  • 阳性对照
    • Extracts from overnight nocodazole treated Hela cells.



Our Abpromise guarantee covers the use of ab39068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/300.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.


  • 功能Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • 组织特异性Placenta and colon.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • 发展阶段Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • 翻译后修饰Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell cycle regulated protein kinase antibody
    • PLK 1 antibody
    • PLK antibody
    • PLK-1 antibody
    • plk1 antibody
    • PLK1_HUMAN antibody
    • Polo like kinase 1 antibody
    • Polo-like kinase 1 antibody
    • Serine/threonine protein kinase 13 antibody
    • Serine/threonine protein kinase PLK1 antibody
    • Serine/threonine-protein kinase 13 antibody
    • Serine/threonine-protein kinase PLK1 antibody
    • STPK 13 antibody
    • STPK13 antibody
    see all

Anti-PLK1 (phospho T210) antibody [2A3] 图像

  • All lanes : Anti-PLK1 (phospho T210) antibody [2A3] (ab39068) at 1 µg/ml

    Lane 1 : Extracts from untreated Hela cells
    Lane 2 : Extracts from overnight nocodazole treated Hela cells

    Predicted band size : 68 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
  • ab39068 staining chicken B lymphoma cells in prometaphase by ICC/IF. Cells were PFA fixed and permeabilized in 0.15% Triton X-100 prior to blocking in 1% BSA for 1 hour at 4°C. The primary antibody was diluted 1/300 (PBS/1%BSA) and incubated with the sample for 1 hour at 37°C. A Texas Red® conjugated goat polyclonal to mouse, diluted 1/200, was used as the secondary.

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Anti-PLK1 (phospho T210) antibody [2A3] (ab39068)参考文献

This product has been referenced in:
  • Platani M  et al. Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomes. J Cell Biol 210:45-62 (2015). ICC/IF ; Human . Read more (PubMed: 26124292) »
  • Kachaner D  et al. Interdomain allosteric regulation of Polo kinase by Aurora B and Map205 is required for cytokinesis. J Cell Biol 207:201-11 (2014). Read more (PubMed: 25332165) »

See all 10 Publications for this product

Product Wall

Thank you for your inquiry.

While we do not list this as cross reacting with mouse, it is possibnle that it may as the regions are so highly conserved. We generally do not recommend using an antibody created in mouse on mouse tissue because ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (Colon carcinoma)
Specification Colon carcinoma
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Eleni Petsalaki

Verified customer

提交于 Dec 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Ms. AeLee Jeong

Verified customer

提交于 Sep 25 2012

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 50 µg
Specification Hela
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jul 23 2012

I am very pleased to hear you would like to accept our offer and test ab39068 in MEFs on Western Blot. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for MEFs on Western Blot ...

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Thank you very much for your interest in ab39068.

To our knowledge, this has not been tested in Western blot on mouse ebryonic fibroblasts (MEFs). Therefore, I can offer a discount off a future purchase if you buy ab39068 now, test it in W...

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The immunogen used for this product has 100% homology with the mouse protein ( This product has not yet been tested in this species however. Because this has been raised ...

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