概述

  • 产品名称
  • 描述
    兔多克隆抗体to PLK1
  • 特异性
    PLK1 Antibody detects endogenous levels of total PLK1 protein.
  • 经测试应用
    适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human PLK1. The antiserum was produced against synthesized peptide derived from human PLK1.
    Database link: P53350

  • 阳性对照
    • Extracts from LOVO cells treated with PMA (125ng/ml, 30mins).

性能

应用

Our Abpromise guarantee covers the use of ab61118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • 组织特异性
    Placenta and colon.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • 发展阶段
    Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • 翻译后修饰
    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • 细胞定位
    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell cycle regulated protein kinase antibody
    • PLK 1 antibody
    • PLK antibody
    • PLK-1 antibody
    • plk1 antibody
    • PLK1_HUMAN antibody
    • Polo like kinase 1 antibody
    • Polo-like kinase 1 antibody
    • Serine/threonine protein kinase 13 antibody
    • Serine/threonine protein kinase PLK1 antibody
    • Serine/threonine-protein kinase 13 antibody
    • Serine/threonine-protein kinase PLK1 antibody
    • STPK 13 antibody
    • STPK13 antibody
    see all

图片

  • Immunofluorescent analysis of A549 cells labeling PLK1 with ab61118 at 1/100 dilution. The picture on the right is blocked with the synthesized peptide.

  • All lanes : Anti-PLK1 antibody (ab61118) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells treated with PMA (125ng/ml, 30mins)
    Lane 2 : Extracts from LOVO cells treated with PMA (125ng/ml, 30mins) with immunising PLK1 peptide


    Predicted band size : 68 kDa
    Observed band size : 68 kDa

    Secondary antibody anti-rabbit HRP (ab6721)

文献

ab61118 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification
Hippocampal homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Feb 12 2014

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