Anti-PLK1抗体[36-298] (ab17057)


  • 产品名称
    参阅全部 PLK1 一抗
  • 描述
    小鼠单克隆抗体[36-298] to PLK1
  • 宿主
  • 经测试应用
    适用于: Indirect ELISA, IP, WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Human PLK1. His-PLK1 full length purified from Sf9 cells.
    Database link: P53350

  • 表位
  • 阳性对照
    • WB: HEK-293, HeLa S3 or U-2 OS cell lysate ICC/IF: HeLa S3, NIH/3T3 or U-2 OS cells Flow Cytometry: U-2 0S cells
  • 常规说明

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab17057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 68 kDa).
ICC/IF 1/200. PubMed: 19033445
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • 功能
    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • 组织特异性
    Placenta and colon.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • 发展阶段
    Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • 翻译后修饰
    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • 细胞定位
    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell cycle regulated protein kinase antibody
    • PLK 1 antibody
    • PLK antibody
    • PLK-1 antibody
    • plk1 antibody
    • PLK1_HUMAN antibody
    • Polo like kinase 1 antibody
    • Polo-like kinase 1 antibody
    • Serine/threonine protein kinase 13 antibody
    • Serine/threonine protein kinase PLK1 antibody
    • Serine/threonine-protein kinase 13 antibody
    • Serine/threonine-protein kinase PLK1 antibody
    • STPK 13 antibody
    • STPK13 antibody
    see all


  • All lanes : Anti-PLK1 antibody [36-298] (ab17057)

    Lane 1 : Recombinant PLK1
    Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell extract
    Lane 3 : HeLa S3 cell extract

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 66 kDa (why is the actual band size different from the predicted?)

    10% SDS-PAGE gel.

  • Immunofluoresence using ab17057 and either HeLa S3, NIH/3T3 (Mouse embyro fibroblast cell line) or U-2 OS (Human bone osteosarcoma epithelial cell line) cells.

  • Anti-PLK1 antibody [36-298] (ab17057) at 1 µg/ml + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

    Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 66 kDa (why is the actual band size different from the predicted?)

    Exposure time: 2 minutes
  • In panel one HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were stained with ab17057 (green) and DAPI. In the second panel, cells were stained with ab17057 (green) and SH-CREST (red), which stains the centromeres. Fix 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeabilized for 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4°C diluted 1/400 in 5% milk in TBST. Secondary antibody incubated 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen.

    Notes: Ample washing between each step.

    TBST = Tris buffered saline + 0.1% Tween. PEM = 80 mM K-PIPES, pH 6.8, 5 mM EGTA, 2 mM MgCl2.

  • Overlay histogram showing U-2 0S (Human bone osteosarcoma epithelial cell line) cells stained with ab17057 (red line). The cells were fixed with 80% methanol (10 minutes) and then permeabilized with 0.1% PBS-Triton X-100 for 15 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab17057, 1 µg/1 x 106 cells) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150117) at 1/2000 dilution for 30 minutes at 22°C.

    Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (ab170190, 1 µg/1 x 106 cells) used under the same conditions.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.


This product has been referenced in:
  • Kim JH  et al. Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation. Biochim Biophys Acta 1863:1307-18 (2016). WB . Read more (PubMed: 27033521) »
  • O'Connor A  et al. Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint. Biol Open 5:11-9 (2015). ICC/IF ; Human . Read more (PubMed: 26685311) »

See all 12 Publications for this product


Western blot
Human Cell lysate - whole cell (HeLa cell)
Gel Running Conditions
Non-reduced Denaturing (9%)
Loading amount
10 µg
Thymidine+RO-3306 or Nocodazole arrest
HeLa cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Yeongmi Cheon

Verified customer

提交于 Jun 07 2017

Thank you for contacting us and sorry for the delay in getting back to you.

As requested, I have looked into the sequence homology between the immunogen used to raise antibodies mentioned and the relevant canineprotein sequence. I have to men...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (rectal cancer)
rectal cancer
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Blocking step
Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks. as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

提交于 Jul 15 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - PBS TritonX100 (0.5%;10min)

Dr. Kirk Mcmanus

Verified customer

提交于 Jun 28 2007

Thank you for your enquiry. Following discussion with our lab I have determined that the reactivity of this antibody against the phospho- or non-phosphorylated forms of Plk1 has unfortunately not been determined. Should you decide to go ahead an...

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Thank you for your enquiry. According to the EXPASY database PLK1 has been conformed to be expressed in placenta and colon tissue in humans and newborn and adult spleen, fetal and newborn kidney, liver, brain, thymus and adult bone marrow, thymus,...

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I just wanted to let you know that I was able to obtain some additional information regarding the IF testing with ab17057. For PLK1 staining they usually fixed the cells with PTEMF Buffer according to Kapoor et al, JCB150(5) 2000, 975-988. He mention...

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Thank you for your enquiry. Regarding the IF/ICC images that we have on the online datasheet, at this time we don't have additional details regarding how the cells were fixed. I suspect that fixation with 4% formaldehyde or cold methanol would be ok. I...

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