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His-PLK1 full length purified from Sf9 cells.
This antibody clone is manufactured by Abcam.
Our Abpromise guarantee covers the use of ab17057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||1/200. PubMed: 19033445|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Immunofluoresence using ab17057 and either HeLaS3, NIH 3T3 or U2OS cells.
Western blot using ab17057.
Lane1: recombinant Plk1
Lane 2: U2OS cell extract
Lane 3: HeLaS3 cell extract
10% SDS-PAGE gel.
Overlay histogram showing U20S cells stained with ab17057 (red line). The cells were fixed with 80% methanol (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab17057, 1 µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150117) at 1/2000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (ab170190, 1 µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.