Anti-PLGF抗体- N-terminal (ab9542)
Key features and details
- Rabbit polyclonal to PLGF - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-PLGF抗体- N-terminal
参阅全部 PLGF 一抗 -
描述
兔多克隆抗体to PLGF - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human PLGF (N terminal). Highly purified N-terminal 20 aa synthetic peptide (Human).
Database link: P49763 -
常规说明
According to Swissprot P49763; PLGF exists in three isoforms PLGF 1, 2 and 3. The N-terminal immunogen sequence of ab9542 is present in all isoforms so extra bands might be observed near the band of interest.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 - 4 µg/ml.
It will detect 25 - 50 ng/lane of recombinant human PLGF-1 (17-18 kDa) and PLGF-2 (22-23 kDa) under reducing conditions. Dimers are detected at higher protein amounts/lane. |
ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 4 µg/ml. It will detect 25 - 50 ng/lane of recombinant human PLGF-1 (17-18 kDa) and PLGF-2 (22-23 kDa) under reducing conditions. Dimers are detected at higher protein amounts/lane. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
组织特异性
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
结构域
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
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数据库链接
- Entrez Gene: 5228 Human
- Omim: 601121 Human
- SwissProt: P49763 Human
- Unigene: 252820 Human
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别名
- D12S1900 antibody
- Pgf antibody
- PGFL antibody
see all
图片
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Western blot of chromatography purified, recombinant PLGF-1/-2 and VEGF165 immobilized to PVDF-membrane.
PlGF antibody shows no cross-reactivity to VEGF165. -
ICC/IF image of ab9542 stained HepG2 (human liver hepatocellular carcinoma cell line) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9542, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
实验方案
数据表及文件
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Datasheet download
文献 (21)
ab9542 被引用在 21 文献中.
- Kang CM et al. Distinctive cytokine profiles of stem cells from human exfoliated deciduous teeth and dental pulp stem cells. J Dent Sci 17:276-283 (2022). PubMed: 35028048
- Carnevale L et al. Celiac Vagus Nerve Stimulation Recapitulates Angiotensin II-Induced Splenic Noradrenergic Activation, Driving Egress of CD8 Effector Cells. Cell Rep 33:108494 (2020). PubMed: 33326772
- Goveia J et al. An Integrated Gene Expression Landscape Profiling Approach to Identify Lung Tumor Endothelial Cell Heterogeneity and Angiogenic Candidates. Cancer Cell 37:21-36.e13 (2020). PubMed: 31935371
- Jiao W et al. Distinct downstream signaling and the roles of VEGF and PlGF in high glucose-mediated injuries of human retinal endothelial cells in culture. Sci Rep 9:15339 (2019). PubMed: 31653890
- Balser C et al. Co-inhibition of PGF and VEGF blocks their expression in mononuclear phagocytes and limits neovascularization and leakage in the murine retina. J Neuroinflammation 16:26 (2019). PubMed: 30732627