Anti-PLGF抗体[37203.111] (ab10966)

概述

  • 产品名称Anti-PLGF抗体[37203.111]
    参阅全部 PLGF 一抗
  • 描述
    小鼠单克隆抗体[37203.111] to PLGF
  • 特异性In immunoblotting, the antibody shows no cross-reactivity with recombinant human VEGF, PDGFAA, PDGFAB, and PDGFBB.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human), expressed in Escherichia coli.

  • 阳性对照
    • Human umbilical vein endothelial cells.
  • 常规说明Do not store in frost-free freezer. Endotoxin level is <10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.

性能

应用

Our Abpromise guarantee covers the use of ab10966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: For capture ELISAs, this product can be used as the capture antibody. Use at a working concentration of 2 µg/ml (100 µl/well) in combination with a biotinylated PlGF polyclonal detection antibody at 100 ng/ml (100 µl/well).
    WB: Use at a concentration of 1 - 2 µg/ml. The detection limit for recombinant human placental growth factor is approximately 20 ng/lane and 300 ng/lane under non-reducing and reducing conditions, respectively. Homodimeric recombinant PlGF migrates as an approximately 29 kDa protein.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
    • 组织特异性While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
    • 序列相似性Belongs to the PDGF/VEGF growth factor family.
    • 结构域Isoform PlGF-2 contains a basic insert which acts as a cell retention signal.
    • 翻译后修饰N-glycosylated.
    • 细胞定位Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • D12S1900 antibody
      • Pgf antibody
      • PGFL antibody
      • PIGF antibody
      • Placenta growth factor antibody
      • Placental growth factor antibody
      • Placental growth factor, vascular endothelial growth factor related protein antibody
      • PlGF 2 antibody
      • PlGF antibody
      • PLGF_HUMAN antibody
      • PlGF2 antibody
      • SHGC 10760 antibody
      see all

    Anti-PLGF antibody [37203.111] (ab10966)参考文献

    ab10966 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for getting back to me and I'm happy to hear that ab10966 worked well for you. We would be very grateful for you if you could submit an Abreview via the product datasheet. We always encourage customers to feed their results back to us, whe...

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    Thank you for your email. I have arranged for a free of charge replacement vial of ab10966 to be sent to you on order# 77020. Lot 95559 is the only batch that we currently have in stock. It is a possibility that your previous vial had gone off during t...

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    Thank you for your email. At this point I would like to offer you a replacement vial free of charge or a refund, please let me know which you would prefer. Thanks again, and I look forward to hearing from you.

    Thank you for your email. I'm replying to with regard to this issue as my colleague Tanya is away for the Easter holidays. With the HUVEC lysate, what dilutions of ab10966 did you try? Did you incubate overnight at 4C? I apologize if this information w...

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    Thank you for contacting us and for providing details of your Western blot protocol. We are very sorry to hear that you are having problem with this antibody. We have read through your message and would like to make the following suggestions/commen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"