概述

  • 产品名称Anti-Plexin D1抗体
    参阅全部 Plexin D1 一抗
  • 描述
    兔多克隆抗体to Plexin D1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to amino acids 1633-1694 (RKKLNTLAHY KIPEGASLAM SLIDKKDNTL GRVKDLDTEK YFHLVLPTDE LAEPKKSHRQ SH) of Human Plexin D1.

  • 阳性对照
    • HepG2 whole cell lysates and HeLa cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: PC12

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 1 x PBS, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 212 kDa.

靶标

Anti-Plexin D1 antibody 图像

  • Anti-Plexin D1 antibody (ab96313) at 1/1000 dilution + HepG2 whole cell lysates at 30 µg

    Predicted band size : 212 kDa
  • Anti-Plexin D1 antibody (ab96313) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 212 kDa
    5% SDS PAGE
  • ab96313 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96313 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Plexin D1 antibody (ab96313)参考文献

ab96313 has not yet been referenced specifically in any publications.

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