ab28762 at 1/300 dilution, staining Plexin D1 in mouse brain tissue sections by IHC-P (Formalin / PFA fixed prafaffin embedded sections). Tissue underwent formaldehyde fixation, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocked in 5% serum for 1 hour. The primary antibody was used at 1/300 dillution for 1 hour. A Biotin-conjugated rabbit polyclonal to goat IgG was used as secondary at 1/250 dillution.
Anti-Plexin D1 antibody (ab28762)参考文献
This product has been referenced in:
Parikh T et al. Proteomic analyses of corneal tissue subjected to alkali exposure. Invest Ophthalmol Vis Sci52:1819-31 (2011).
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Sakurai A et al. Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras. Mol Cell Biol30:3086-98 (2010).
WB, ICC/IF, IP
Human, Mouse, African green monkey
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