概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab28762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20385769
IP Use at an assay dependent concentration. PubMed: 20385769
WB 1/1000.
IHC-P 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-Plexin D1 antibody 图像

  • ab28762 at 1/300 dilution, staining Plexin D1 in mouse brain tissue sections by IHC-P (Formalin / PFA fixed prafaffin embedded sections). Tissue underwent formaldehyde fixation, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocked in 5% serum for 1 hour. The primary antibody was used at 1/300 dillution for 1 hour. A Biotin-conjugated rabbit polyclonal to goat IgG was used as secondary at 1/250 dillution. 

Anti-Plexin D1 antibody (ab28762)参考文献

This product has been referenced in:
  • Parikh T  et al. Proteomic analyses of corneal tissue subjected to alkali exposure. Invest Ophthalmol Vis Sci 52:1819-31 (2011). WB . Read more (PubMed: 20861482) »
  • Sakurai A  et al. Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras. Mol Cell Biol 30:3086-98 (2010). WB, ICC/IF, IP ; Human, Mouse, African Green Monkey . Read more (PubMed: 20385769) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Artherial endothelial cells)
Loading amount 20 µg
Specification Artherial endothelial cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Elena Astanina

Verified customer

提交于 Sep 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse Brain)
Specification Mouse Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Manoj Kumar Valluru

Verified customer

提交于 Sep 04 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"