Anti-Plexin B1抗体(ab39717)

概述

  • 产品名称Anti-Plexin B1抗体
    参阅全部 Plexin B1 一抗
  • 描述
    兔多克隆抗体to Plexin B1
  • 特异性PLexin B1 is readily cleaved at the intra- extra- cellular boundary (circa aa.1305/1306) likely giving rise to the several different band sizes routinely observed in WB, depending on the isoform that has been cleaved.
  • 经测试应用适用于: IHC-FoFr, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Plexin B1.

    (Peptide available as ab41062.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Mouse Liver Rat Liver Rat Pancreas

性能

应用

Our Abpromise guarantee covers the use of ab39717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/1000.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa).

靶标

  • 相关性Plexin B1 is the transmembrane receptor for the class 4 semaphorin, SEMA4D. Plexin B1 activation plays a role in axon guidance, invasive growth and cell migration and has been reported to have additional roles in RHOA activation and subsequent changes of the actin cytoskeleton.
  • 细胞定位Secreted and single pass type I membrane protein.
  • 数据库链接
  • 别名
    • KIAA0407 antibody
    • Plexin 5 antibody
    • Plexin B1 antibody
    • PLXN5 antibody
    • PLXNB1 antibody
    • Semaphorin receptor antibody
    • Semaphorin receptor SEP antibody
    • SEP antibody
    see all

Anti-Plexin B1 antibody 图像

  • All lanes : Anti-Plexin B1 antibody (ab39717) at 2 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 233 kDa
    Observed band size : 171 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 155 kDa. We are unsure as to the identity of these extra bands.
    We believe that the band observed at 171 kDa corresponds to a cleaved form of Plexin B1.
  • Immunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100.  Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).

Anti-Plexin B1 antibody (ab39717)参考文献

ab39717 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Primary dissection of hippocampal neurons.)
Specification Primary dissection of hippocampal neurons.
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Michael Weible

Verified customer

提交于 Nov 08 2012



Expiration date: 2012-11-09


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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
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Dr. Sophie Pezet

Verified customer

提交于 Feb 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"