概述

  • 产品名称
    Anti-Plexin A4抗体
    参阅全部 Plexin A4 一抗
  • 描述
    兔多克隆抗体to Plexin A4
  • 经测试应用
    适用于: IHC-FoFr, WB, ICC/IF, IHC-FrFlmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse Plexin A4.

    (Peptide available as ab39349.)

  • 阳性对照
    • ab39350 gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Mouse Brain (P0); Mouse Dorsal Root Ganglia.

性能

应用

Our Abpromise guarantee covers the use of ab39350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 213 kDa (predicted molecular weight: 213 kDa).Can be blocked with Mouse Plexin A4 peptide (ab39349).
ICC/IF Use a concentration of 1 µg/ml.
IHC-FrFl Use at an assay dependent concentration.

靶标

图片

  • All lanes : Anti-Plexin A4 antibody (ab39350) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/35000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 213 kDa
    Observed band size : 213 kDa
    Additional bands at : 190 kDa,41 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab39350 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-Plexin A4 antibody (ab39350) at 1/250 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 3 : Dorsal Root Ganglia (Mouse) Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
    Lane 5 : Mouse brain tissue lysate - total protein (0 days) (ab7188) with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
    Lane 6 : Dorsal Root Ganglia (Mouse) Tissue Lysate with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
    Lanes 3 - 6 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution


    Performed under reducing conditions.

    Predicted band size : 213 kDa

    We are unsure as to the biological basis of the band at 190 kDa. However, it is successfully blocked by peptide (ab39349) suggesting this may be a possible isoform of Plexin A4.

  • ICC/IF image of ab39350 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39350, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • ab39350 staining Plexin A4 in mouse brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.

    See Abreview

文献

This product has been referenced in:
  • Lee FH  et al. Disrupting GluA2-GAPDH Interaction Affects Axon and Dendrite Development. Sci Rep 6:30458 (2016). IHC-P ; Mouse . Read more (PubMed: 27461448) »
  • Yamashita N  et al. Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic localization of GluA2-containing AMPA receptors. Nat Commun 5:3424 (2014). Read more (PubMed: 24599038) »

See all 7 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Tris pH9.0 + 1mM EDTA
Permeabilization
No
Specification
Pancreas
Fixative
Formaldehyde
Username

Dr. Yves Heremans

Verified customer

提交于 Jun 30 2017

Thank you for your reply.

I am sorry to hear that the replacement antibody didn't work for you. This is the only lot in stock and we do not plan on making a new batch until we sell the 15 vials we have in stock.

I would be happy to ...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Thank you for contacting us.

Anja is on her well deserved holiday, therefore, I will deal with your case.

It is very possible that Gutekunstet al. used another lot: so far, we have produced more than 50 lots of this antibody. We ta...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

提交于 Aug 18 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 17 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cultured Cells (dissociated cortical neurons)
Specification
dissociated cortical neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton X
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Dr. Ioana Carcea

Verified customer

提交于 Apr 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册