概述

  • 产品名称Anti-Plexin A4抗体
    参阅全部 Plexin A4 一抗
  • 描述
    兔多克隆抗体to Plexin A4
  • 经测试应用适用于: IHC-FoFr, WB, ICC/IF, IHC-FrFlmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse Plexin A4.

    (Peptide available as ab39349.)

  • 阳性对照
    • ab39350 gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Mouse Brain (P0); Mouse Dorsal Root Ganglia.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab39350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr
WB
ICC/IF
IHC-FrFl
  • 应用说明ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-FoFr: 1/1000 (See abreview).
    WB: 1/250. Detects a band of approximately 213 kDa (predicted molecular weight: 213 kDa). Can be blocked with Plexin A4 peptide (ab39349).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Plexin A4 antibody 图像

    • All lanes : Anti-Plexin A4 antibody (ab39350) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate, blocked with 3% milk
      Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
      Lane 3 : Brain (Rat) Tissue Lysate, blocked with 3% milk

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/35000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 213 kDa
      Observed band size : 213 kDa
      Additional bands at : 190 kDa,41 kDa,70 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 8 minutes

      This western blot used the Optiblot ECL Detect Kit (ab133406). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.



    • Performed under reducing conditions.

      Predicted band size : 213 kDa
      We are unsure as to the biological basis of the band at 190 kDa. However, it is successfully blocked by peptide (ab39349) suggesting this may be a possible isoform of Plexin A4.
    • ICC/IF image of ab39350 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39350, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
    • ab39350 staining Plexin A4 in mouse brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.

      See Abreview

    Anti-Plexin A4 antibody (ab39350)参考文献

    This product has been referenced in:
    • Yamashita N  et al. Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic localization of GluA2-containing AMPA receptors. Nat Commun 5:3424 (2014). Read more (PubMed: 24599038) »
    • Mlechkovich G  et al. Distinct cytoplasmic domains in Plexin-A4 mediate diverse responses to semaphorin 3A in developing mammalian neurons. Sci Signal 7:ra24 (2014). Read more (PubMed: 24619647) »

    See all 6 Publications for this product

    Product Wall

    Thank you for your reply.

    I am sorry to hear that the replacement antibody didn't work for you. This is the only lot in stock and we do not plan on making a new batch until we sell the 15 vials we have in stock.

    I would be happy to ...

    Read More

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More

    Thank you for contacting us.

    Anja is on her well deserved holiday, therefore, I will deal with your case.

    It is very possible that Gutekunstet al. used another lot: so far, we have produced more than 50 lots of this antibody. We ta...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry free floating
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Username

    Abcam user community

    Verified customer

    提交于 Aug 18 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Brain sections)
    Specification Brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Mar 17 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cultured Cells (dissociated cortical neurons)
    Specification dissociated cortical neurons
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% triton X
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Dr. Ioana Carcea

    Verified customer

    提交于 Apr 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"