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Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse Plexin A4.
(Peptide available as ab39349.)
Our Abpromise guarantee covers the use of ab39350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This western blot used the Optiblot ECL Detect Kit (ab133406). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab39350 staining Plexin A4 in mouse brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.