概述

  • 产品名称Anti-Plexin A3抗体
    参阅全部 Plexin A3 一抗
  • 描述
    兔多克隆抗体to Plexin A3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性

    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide derived from within residues 1100 - 1200 of Mouse Plexin A3.

    (Peptide available as ab41563.)

  • 阳性对照
    • This antibody gave a positive signal in P0 mouse brain and mouse hippocampus tissue lysates

性能

应用

Our Abpromise guarantee covers the use of ab41564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性Plexin A3 is a coreceptor for the class 3 secreted semaphorins. This family of semaphorins binds to a complex composed of a neuropilin and a plexin. The plexin type modulates the affinity of the complex for specific semaphorins and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Plexin A3 is a putative receptor involved in the development of neural and epithelial tissues.
  • 细胞定位Membrane; single-pass type I membrane protein.
  • 数据库链接
  • 别名
    • HSSEXGENE antibody
    • Plexin 4 antibody
    • PLXN4 antibody
    • PLXNA3 antibody
    • Semaphorin receptor SEX antibody
    • SEX antibody
    • Transmembrane protein sex antibody
    • XAP 6 antibody
    see all

Anti-Plexin A3 antibody 图像

  • All lanes : Anti-Plexin A3 antibody (ab41564) at 1 µg/ml

    Lane 1 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 2 : Hippocampus (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 207 kDa
    Observed band size : 207 kDa
    Additional bands at : 32 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Plexin A3 antibody (ab41564)参考文献

This product has been referenced in:
  • Demyanenko GP  et al. Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling. J Neurosci 34:11274-87 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 25143608) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Cancer cells)
Loading amount 22 µg
Specification Cancer cells
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Manoj Kumar Valluru

Verified customer

提交于 Jul 23 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E 14 Mouse Embryo)
Specification E 14 Mouse Embryo
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Manoj Kumar Valluru

Verified customer

提交于 Nov 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"