概述

  • 产品名称Anti-Plexin A2抗体
    参阅全部 Plexin A2 一抗
  • 描述
    兔多克隆抗体to Plexin A2
  • 经测试应用适用于: WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human不与反应: Rat
  • 免疫原

    Synthetic peptide derived from within residues 300 - 400 of Mouse Plexin A2.

    (Peptide available as ab39356.)

  • 阳性对照
    • This antibody gave a positive signal in Mouse Brain (0 Day) Tissue Lysate

性能

应用

Our Abpromise guarantee covers the use of ab39357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 212 kDa).
IHC-FoFr 1/300.

靶标

  • 功能Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
  • 组织特异性Detected in fetal brain.
  • 序列相似性Belongs to the plexin family.
    Contains 4 IPT/TIG domains.
    Contains 1 Sema domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Plexin-A2 antibody
    • PlexinA2 antibody
    • PLXA2 antibody
    • PLXA2_HUMAN antibody
    • PLXNA2 antibody
    • Semaphorin receptor OCT antibody
    see all

Anti-Plexin A2 antibody 图像

  • Anti-Plexin A2 antibody (ab39357) at 2 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Secondary
    Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 212 kDa
    Observed band size : 230 kDa (why is the actual band size different from the predicted?)

    Plexin A2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

  • IHC-FoFr image of Plexin A2 staining on mouse cortex (Lamina V) using ab39357 (1/300). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

  • Immunofluorescence analysis of mouse neural tube cells, staining Plexin A2 (green) with ab39357.

    Cells were fixed before being blocked with 10% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

Anti-Plexin A2 antibody (ab39357)参考文献

This product has been referenced in:
  • Sabag AD  et al. The role of the plexin-A2 receptor in Sema3A and Sema3B signal transduction. J Cell Sci 127:5240-52 (2014). Read more (PubMed: 25335892) »
  • Li W  et al. Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development. Proc Natl Acad Sci U S A 110:1738-43 (2013). Read more (PubMed: 23319608) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neural Tube)
Specification Neural Tube
Fixative IF-P
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Nov 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Feb 08 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (embryo d15 spinal cord, kidney, adult brain)
Specification embryo d15 spinal cord, kidney, adult brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"