RabMAb

Anti-Plexin A1抗体[EPR3449] (ab92346)

概述

  • 产品名称Anti-Plexin A1抗体[EPR3449]
    参阅全部 Plexin A1 一抗
  • 描述
    兔单克隆抗体[EPR3449] to Plexin A1
  • 特异性ab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally.
  • 经测试应用适用于: WB, ICC, Flow Cytmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Plexin A1.

  • 阳性对照
    • KS62, HUVEC and HepG2 cell lysates; fetal brain tissue lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab92346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC
Flow Cyt
  • 应用说明Flow Cyt: 1/50.
    ICC: 1/100.
    WB: 1/1000 - 1/2000. Predicted molecular weight: 211 kDa.

    Is unsuitable for IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Plexin A1 antibody [EPR3449] 图像

    • All lanes : Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Fetal brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 211 kDa

    Anti-Plexin A1 antibody [EPR3449] (ab92346)参考文献

    ab92346 has not yet been referenced specifically in any publications.

    Product Wall

    Gracias por tu respuesta.

    Me temo que no puedo mandarte ambos anticuerpos, pero en caso de que el nuevo vial que te enviemos no te funcionara tampoco, éste también estaría cubierto por la garantía Abpromise, y por ...

    Read More

    Gracias por enviarnos los resultados.
    Si te interesa puedo enviarte otro vial de un lote diferente, o incluso un vial de otro anti-Plexin A1, el ab32960:
    http://www.abcam.com/Plexin-A1-antibody-ab32960.html
    A pesar de que la garantía ha ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Spleen)
    Loading amount 20 µg
    Specification Spleen
    Gel Running Conditions Reduced Denaturing (gel 7,5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 16 2012

    Thank you for contacting Abcam.   I have asked the lab to provide the protocol that they used to test ab92346 in flow cytometry is attached(also attached is the data from the lab). They also informed that ab92346 recognizes the extracellular domain of ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"