重组Anti-Plexin A1抗体[EPR3449] (ab92346)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3449] to Plexin A1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Plexin A1抗体[EPR3449]
参阅全部 Plexin A1 一抗 -
描述
兔单克隆抗体[EPR3449] to Plexin A1 -
宿主
Rabbit -
特异性
ab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally. -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Plexin A1 aa 100-200. The exact sequence is proprietary.
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阳性对照
- WB: KS62, HUVEC and HepG2 whole cell lysate (ab7900); fetal brain tissue lysate Flow Cyt (intra): K562 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
This antibody is not purified. It is provided in cell supernatant and storage buffer. -
克隆
单克隆 -
克隆编号
EPR3449 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92346于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/1000 - 1/2000. Predicted molecular weight: 211 kDa.
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说明 |
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Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Predicted molecular weight: 211 kDa. |
靶标
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相关性
Function: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Tissue specificity: Detected in fetal brain, lung, liver and kidney. Similarity: Belongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain. -
细胞定位
Cell Membrane -
数据库链接
- Entrez Gene: 5361 Human
- Entrez Gene: 18844 Mouse
- NCBI: NP_115618 Human
- Omim: 601055 Human
- SwissProt: Q9UIW2 Human
- SwissProt: P70206 Mouse
- Unigene: 432329 Human
- Unigene: 3789 Mouse
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别名
- NOV antibody
- NOV/plexin A1 protein antibody
- NOVP antibody
see all
图片
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All lanes : Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 211 kDa -
Intracellular Flow Cytometry analysis of permeabilized K562 cells labeling Plexin A1 with ab92346 at 1/50 (red) campared to a rabbit IgG (negative control) (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab92346 被引用在 1 文献中.
- Wu JH et al. Semaphorin-3A protects against neointimal hyperplasia after vascular injury. EBioMedicine 39:95-108 (2019). PubMed: 30579864