概述

  • 产品名称
    Anti-Plexin A1抗体
    参阅全部 Plexin A1 一抗
  • 描述
    兔多克隆抗体to Plexin A1
  • 经测试应用
    适用于: WB, ELISA, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Plexin A1 aa 182-200.
    Sequence:

    RLMANEEDADMFGFVYQDE

  • 阳性对照
    • Cos 7 cells transfected with mouse Plexin A1 or neonatal rat brain.

性能

应用

Our Abpromise guarantee covers the use of ab23391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 195 kDa.
ELISA 1/2000.
IP 1/50 - 1/200.
ICC/IF 1/200. (see Abreview).

靶标

  • 相关性
    Function: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Tissue specificity: Detected in fetal brain, lung, liver and kidney. Similarity: Belongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain.
  • 细胞定位
    Cell Membrane
  • 数据库链接
  • 别名
    • NOV antibody
    • NOV/plexin A1 protein antibody
    • NOVP antibody
    • Plexin-A1 antibody
    • Plexin1 antibody
    • PlexinA1 antibody
    • PLXA1_HUMAN antibody
    • PLXN1 antibody
    • Plxna1 antibody
    • PLXNA1 protein antibody
    • Semaphorin receptor NOV antibody
    see all

图片



  • Predicted band size : 195 kDa

文献

This product has been referenced in:
  • Delloye-Bourgeois C  et al. PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments. Nat Neurosci 18:36-45 (2015). Read more (PubMed: 25485759) »
  • Hernandez-Enriquez B  et al. Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing. Genes Dev 29:2617-32 (2015). Read more (PubMed: 26680304) »

See all 8 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Tris pH9.0 + 1mM EDTA
Permeabilization
No
Specification
Pancreas
Fixative
Formaldehyde
Username

Dr. Yves Heremans

Verified customer

提交于 Jun 21 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (dendritic cells)
Specification
dendritic cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. So-Young Eun

Verified customer

提交于 Jun 11 2007

Thank you for contacting us. For ab23391, the blocking peptide is ab42757. For ab25999, the blocking peptide is ab42770. If you have any other questions, please do not hesitate to contact us.

Thank you for your enquiry. This antibody was tested for Plexin-A1 reactivity using transfected cells. It was not tested against other Plexin family members. However, the immunogen was chosen based on low homology to other family members. The only s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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