概述

  • 产品名称Anti-PLEKHO1抗体
    参阅全部 PLEKHO1 一抗
  • 描述
    小鼠多克隆抗体to PLEKHO1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-409 of Human PLEKHO1 (NP_057358.2)

  • 阳性对照
    • Hela cell lysate, transfected 293T cells

性能

应用

Our Abpromise guarantee covers the use of ab89110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.

靶标

  • 功能Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.
  • 组织特异性Abundantly expressed in skeletal muscle and heart, moderately in kidney, liver, brain and placenta and sparingly in the pancreas and lung. Easily detectable in cancer cell lines such as MOLT-4, HEK293 and Jurkat cells.
  • 序列相似性Contains 1 PH domain.
  • 翻译后修饰C-terminal fragments could be released during apoptosis via caspase-3-dependent cleavage.
  • 细胞定位Cell membrane. Nucleus. Cytoplasm. Predominantly localized to the plasma membrane. In C2C12 cells, with the absence of growth factor, it is found in the nucleus. It rapidly translocates to the plasma membrane after insulin stimulation. In response to TNF, it translocates from the plasma membrane to the cytoplasm and then to the nucleus accompanied by cleavage by caspase-3. However, the subcellular location is highly dependent of the cell type, and this explains why it is found exclusively at the plasma membrane, in some type of cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-Jun-binding protein antibody
    • Casein kinase 2-interacting protein 1 antibody
    • CK2 interacting protein 1 antibody
    • CK2 interacting protein 1 HQ0024c protein antibody
    • CK2-interacting protein 1 antibody
    • CKIP 1 antibody
    • CKIP-1 antibody
    • CKIP1 antibody
    • JBP antibody
    • OC120 antibody
    • Osteoclast maturation associated gene 120 protein antibody
    • Osteoclast maturation-associated gene 120 protein antibody
    • PH domain-containing family O member 1 antibody
    • PKHO1_HUMAN antibody
    • Pleckstrin homology domain containing family O member 1 antibody
    • Pleckstrin homology domain-containing family O member 1 antibody
    • PLEKHO1 antibody
    see all

Anti-PLEKHO1 antibody 图像

  • Anti-PLEKHO1 antibody (ab89110) at 1 µg/ml + HeLa lysate at 50 µg

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PLEKHO1 antibody (ab89110) at 1 µg/ml

    Lane 1 : PLEKHO1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of purified ab89110 to PLEKHO1 on HeLa cell. [antibody concentration 10 ug/ml]

Anti-PLEKHO1 antibody (ab89110)参考文献

ab89110 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab89110.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"