概述

  • 产品名称Anti-PLEKHH2抗体
  • 描述
    兔多克隆抗体to PLEKHH2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 1045-1094 (LALCVGLFLP HHPFLWLLRL HLKRNADSRT EFGKYAIYCQ RCVERTQQNG) of Human PLEKHH2 (NP_742066).

  • 阳性对照
    • Jurkat cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab81300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 168 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-PLEKHH2 antibody 图像

  • Anti-PLEKHH2 antibody (ab81300) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 168 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • ab81300 at 10 µg/ml staining PLEKHH2 in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab81300 at 10 µg/ml staining PLEKHH2 in Human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PLEKHH2 antibody (ab81300)参考文献

ab81300 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"