antigen: YDRVDEEVHR LVLTSNNRLQ QLEHLRELAS LLEGNDQQSC QKGLQLAKEN PQRTEEMVQD FRRGLSAVVS QAECREGELA RWTRSSELCE TVS, corresponding to amino acids 437-529 of Human PLEKHG4B (Q96PX9).
- Human corpus, uterine tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Information by UniProt
- PH domain-containing family G member 4B antibody
- PKH4B_HUMAN antibody
- Pleckstrin homology domain-containing family G member 4B antibody
- PLEKHG4B antibody
Immunocytochemistry/ Immunofluorescence - Anti-PLEKHG4B antibody (ab122325)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab122325 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLEKHG4B antibody (ab122325)
Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human corpus, uterine with ab122325 at 1/1000 dilution showing strong cytoplasmic positivity in glandular cells.
has not yet been referenced specifically in any publications.
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