Anti-PLEKHG2抗体(ab68756)

概述

  • 产品名称Anti-PLEKHG2抗体
  • 描述
    小鼠多克隆抗体to PLEKHG2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human), PLEKHG2 (AAH13426.1, 896 a.a).

  • 阳性对照
    • PLEKHG2 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab68756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa.

靶标

  • 功能May be a transforming oncogene with exchange activity for CDC42 (By similarity). May be a guanine-nucleotide exchange factor (GEF) for RAC1 and CDC42. Activated by the binding to subunits beta and gamma of the heterotrimeric guanine nucleotide-binding protein (G protein).
  • 序列相似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110070J07Rik antibody
    • AA673237 antibody
    • CLG antibody
    • FLJ00018 antibody
    • FLJ22458 antibody
    • PH domain-containing family G member 2 antibody
    • PKHG2_HUMAN antibody
    • Pleckstrin homology domain containing family G member 2 antibody
    • Pleckstrin homology domain-containing family G member 2 antibody
    • Plekhg2 antibody
    • ZFYVE18 antibody
    see all

Anti-PLEKHG2 antibody 图像

  • All lanes : Anti-PLEKHG2 antibody (ab68756) at 1/500 dilution

    Lane 1 : PLEKHG2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

Anti-PLEKHG2 antibody (ab68756)参考文献

ab68756 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Skin Fibroblasts)
Loading amount 25 µg
Specification Skin Fibroblasts
Gel Running Conditions Reduced Denaturing (12.5)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Mar 18 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Skin Fibroblasts)
Specification Skin Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

提交于 Dec 13 2012

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